ABSTRACT
Objective. The purpose of this research was to evaluate the digestibility of different nutritional and energy components of artificial diets containing flour fishsilage used in growing of arawana fingerlings (O. bicirrhossum). Materials and methods. 240 fingerlings distributed in 12 tanks with 20 fish per experimental unit were handled, in a completely randomized design made up of 4 treatments with three replicates, each with inclusions of 0 (0ESH), 10 (10ESH), 20(20ESH) and 30% flour fish silage (30ESH) of treatments T0, T1, T2 and T3 respectively; fromflour fish silagein order to formulate isoenergetic and isonitrogenous diets in the different treatments to avoid sources of miscalculation of the experimental variables. Results. High digestibility coefficients were observed in treatments T1 (10ESH) and T3 (30ESH). The protein, lipid and energy utilization ratios were greater than 60%, which shows the importance of including flour fish silage as a source of protein to improve the various growth parameters. Significant differences between treatments were found in the increase of length (p≤0.05), showing that the T1 was the best treatment with an average of 1.21±0.29 cm. Conclusions. According to the coefficients of digestibility, length increase and benefit - cost ratio, the best treatment was T1 (10ESH) used to feed arawana (O. bicirrhossum), during the growing period, demonstrating the economic and ecological advantages for utilization of this source of protein in aquaculture.
Objetivo. Analizar la digestibilidad de los diferentes componentes nutricionales y energéticos en dietas balanceadas con niveles de harina de hidrolizado de vísceras de cachama en el alevinaje de arawana (O. bicirrhossum). Materiales y métodos. Se evaluaron 240 alevinos, distribuidos en 12 acuarios a razón de 1 animal/5 L. Se utilizó un diseño completamente al azar, conformado por cuatro tratamientos con tres réplicas cada uno, con inclusiones de 0 (0ESH), 10 (10ESH), 20 (20ESH) y 30% de extracto seco de hidrolizado de vísceras de cachama (30ESH) para los tratamientos T0, T1, T2 y T3 respectivamente; para evitar fuentes de error en el cálculo de las variables experimentales, se formularon dietas isoenergéticas e isonitrogenadas. Resultados. Se detectaron diferencias estadísticas significativas en el incremento de longitud (p≤0.05), indicando que el T1 (10ESH) es el mejor tratamiento con un promedio de talla de 1.21 ±0.29 cm, además, se obtuvieron coeficientes de digestibilidad superiores al 60% en los tratamientos T1 (10ESH) y T3 (30ESH) en lo referente a proteína, lípidos y Coeficientes de Utilización Energética (CUE); demostrando la importancia de incluir el extracto seco de hidrolizado de vísceras de cachama, en las dietas de los peces para mejorar las distintas variables zootécnicas, teniendo en cuenta la importancia acuariofílica de la especie. Conclusiones. De acuerdo con los coeficientes de digestibilidad, incrementos de longitud y la relación beneficio - costo el mejor tratamiento fue el T1 (10 ESH) en dietas de levante para O. bicirrhossum; demostrando las ventajas económicas y ecológicas de la incorporación de esta materia prima en la alimentación acuícola.
Subject(s)
Diet , Nutritional Sciences , SilageABSTRACT
We evaluated a multiplex-PCR to differentiate Mycobacterium bovis from M. tuberculosis Complex (MTC) by one step amplification based on simultaneous detection of pncA 169C > G change in M. bovis and the IS6110 present in MTC species. Our findings showed the proposed multiplex-PCR is a very useful tool for complementation in differentiating M. bovis from other cultured MTC species.
Subject(s)
Bacteriological Techniques/methods , Molecular Diagnostic Techniques/methods , Multiplex Polymerase Chain Reaction/methods , Mycobacterium bovis/isolation & purification , Mycobacterium tuberculosis/isolation & purification , Tuberculosis/microbiology , Amidohydrolases/genetics , DNA Transposable Elements , DNA, Bacterial/genetics , Mycobacterium bovis/classification , Mycobacterium bovis/genetics , Mycobacterium tuberculosis/classification , Mycobacterium tuberculosis/genetics , Tuberculosis/diagnosisABSTRACT
The objective of this study was to investigate the bone regeneration of a "gold standard" (autograft) from iliac crest associated with cellular therapy in rabbits. A bone defect was created with 10x5x5mm in 28 rabbit mandibles. The control group animals (n=14) were repaired with autograft of iliac crest and the experimental group animals (n=14) received iliac crest autograft in association with mononuclear cells from the bone marrow of the femur. Weekly radiographs were taken of the surgery region and histological analyses was performed in seven animals in each group at 15 days and in seven animals of each group at 30 days after the surgery. A gradual increase of bone density was observed and the experimental animals presented the bone bridge in 85.7 percent (6/7) of the cases, while only 42.8 percent (3/7) of the animals in the control group presented this structure 28 days after the surgery. The histopathological parameters analyzed did not show any statistical difference between the control and experimental group in 15 and 30 days of analysis. The results suggest that the mononuclear cells from the marrow bone can better support the autograft regeneration in mandible defects in rabbits.
Avaliou-se a regeneração óssea de auto-enxerto, considerado "padrão ouro" da crista ilíaca associado à terapia celular da medula óssea em coelhos. Foi criado um defeito ósseo de 10x5x5mm na mandíbula de 28 coelhos, distribuídos em grupo-controle com, 14 animais, reparados com auto-enxerto de crista ilíaca, e grupo experimental com, 14 animais, em que o auto-enxerto foi associado a células mononucleares da medula óssea autógena do fêmur. Foram realizadas radiografias semanais da região operada e análise histológica em sete animais de cada grupo aos 15 e em sete de cada grupo aos 30 dias do pós-operatório. Houve aumento gradativo da densidade óssea, e 85,7 por cento (6/7) dos animais do grupo experimental e 42,8 por cento (3/7) do grupo-controle apresentaram formação de ponte óssea 28 dias após a cirurgia. Na análise histopatológica aos 15 dias, os enxertos foram facilmente visualizados e a atividade das células fagocitárias foi intensa. Já aos 30 dias, a visualização foi mais difícil e, quando possível, apenas um resquício foi visualizado. Os resultados sugerem que a adição de células mononucleares da medula óssea favorece a regeneração do auto-enxerto em defeitos mandibulares de coelhos.
Subject(s)
Animals , Male , Bone Marrow Cells , Bone Regeneration , Ilium/transplantation , Mandible , Rabbits , Transplantation, Autologous/veterinary , Bone Transplantation/veterinary , Bone Density , Cell Separation , Deglutition , MasticationABSTRACT
Background: Ozone therapy may stimulate antioxidant systems and protect against free radicals. It has not been used formerly in patients with pulmonary emphysema. Aim: To assess the effects of rectal ozone therapy in patients with pul-monary emphysema. Material and Methods: Sixty four patients with pulmonary emphysema, aged between 40 and 69 years, were randomly assigned to receive rectal ozone in 20 daily sessions, rectal medicinal oxygen or no treatment. Treatments were repeated three months later in the frst two groups. At baseline and at the end of the study, spirometry and a clinical assessment were performed. Results: fifty patients completed the protocol, 20 receiving ozone therapy, 20 receiving rectal oxygen and 10 not receiving any therapy. At baseline, patients on ozone therapy had significantly lower values of forced expiratory volume in the frst second (fEV1) and fEV1/forced vital capacity. At the end of the treatment period, these parameters were similar in the three treatment groups, therefore they only improved significantly in the group on ozone therapy. No differences were observed in other spirometric parameters. Conclusions: Rectal ozone therapy may be useful in patients with pulmonary emphysema.
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Oxidants, Photochemical/administration & dosage , Oxygen/administration & dosage , Ozone/administration & dosage , Pulmonary Emphysema/therapy , Administration, Rectal , Double-Blind Method , Pulmonary Emphysema/physiopathology , Respiratory Function Tests , Severity of Illness Index , Treatment OutcomeABSTRACT
A qualidade das sementes esta relacionada de forma direta ao seu potencial fisiológico, representado pela germinação e/ou vigor, expressando sua capacidade de originar plântulas normais. O objetivo do estudo foi a análise dos efeitos da temperatura e do tempo de exposição ao teste de envelhecimento acelerado sobre os parâmetros: germinação, germinação na primeira contagem, emergência, Índice de velocidade de emergência, comprimento de parte aérea e massa seca. O ensaio foi conduzido no delineamento inteiramente casualizado, com quatro repetições. As sementes foram submetidas à dois métodos de envelhecimento acelerado: o tradicional com água destilada e outro com solução saturada de 40 g NaCl 100 mL-1 de água, em duas temperaturas (38 ºC e 41 ºC) e em três períodos de exposição (48, 72 e 96 horas). Melhor se pode verificar o potencial fisiológico das sementes de coentro, quando submetido às condições estressantes: na temperatura de 41 °C com o período de exposição de 96 h (Teste envelhecimento acelerado tradicional) e na temperatura de 41 ºC com período de exposição de 48 h (com solução saturada). Os testes de germinação e primeira contagem evidenciaram diferenças na qualidade fisiológica inicial das sementes em função dos tratamentos aplicados.
The quality quality of the seeds this related of direct form to it physiologic potential, acted by the germination and/or energy, expressing it capacity to originate normal plantules. The objective of the study was the analysis of the effects of the temperature and of the time of exhibition to the test of accelerated aging on the parameters: germination, germination in the first counting, emergency, Index of emergency speed, length of aerial part and mass dries. The rehearsal was driven in the delineamento entirely casualizado, with four repetitions. The seeds were submitted to two methods of accelerated aging: the traditional with distilled water and other with saturated solution of 40 g NaCl 100 mL-1 of water, in two temperatures (38 ºC and 41 ºC) and in three exhibition periods (48, 72 and 96 hours). Better the physiologic potential of the cilantro seeds can be verified, when submitted to the stressful conditions: in the temperature of 41 °C with the period of exhibition of 96 h (it Tests traditional aging) and in the temperature of 41 °C with period of exhibition of 48 h (with saturated solution). The germination tests and first counting evidenced differences in the quality physiologic initial of the seeds in function of the applied treatments.
Subject(s)
Seeds/metabolism , Coriandrum/classification , Seeds/growth & development , GerminationABSTRACT
El objetivo de este trabajo es correlacionar el tiempo de protrombina y RIN en dos combinaciones de reactivos e instrumentos de medición en muestras de pacientes pediátricos con insuficiencia hepática asistidos en el Hospital de Pediatría Juan P. Garrahan y pasibles de trasplante. Se estudiaron 62 muestras de sangre obtenidas de pacientes, con presencia de patología hepática, definida con evidencia bioquímica de injuria hepática. Se utilizaron 2 (dos) analizadores, un coagulómetro BCT de detección foto-óptica y un Sta Compact de detección electromagnética. Para el analizador BCT se utilizó el reactivo Thromborel S, en el Sta Compact el Sta Neoplastine. Se trabajó con el ISI correspondiente a cada Reactivo /Instrumento. El análisis estadístico se realizó con el programa informático Stata 9.0, aplicando los tests estadísticos de coeficiente de correlación (rho) y el intervalo de confianza 95%; se determino la ecuación de la recta, para cada caso, se determino la equivalencia clínica a través del EP evaluator. Nuestra conclusión es que en los pacientes con insuficiencia hepática, se halló mejor correlación estadística y clínica para el TP%, debido a que se independiza del Tiempo (seg.) y de la calidad de la tromboplastina (diferentes concentraciones de factor tisular). Según nuestros resultados el sistema ISI/RIN, no seria válido para aplicarlo a pacientes con falla hepática y pasible de trasplante. Una de las causas es la falta de un reactivo de Tromboplastina calibrado con plasmas de pacientes con insuficiencia hepática, ya que para el cálculo del ISI, se usan plasmas de pacientes con terapia ACO (AU)
The objective of this study was to correlate prothrombin time (PT) and INR in two combinations of reagents and measurement instruments samples of pediatric patients with liver failure seen at the Hospital de Pediatría Juan P. Garrahan and who are candidates for liver transplantation. Sixtytwo blood samples were studied obtained from patients with liver pathology defined by biochemical evidence of liver injury. Two analyzers were used: a BCT coagulometer with photo-optical clot detection and a Sta Compact with an electromagnetic detection system. For the BCT analyzer Thromborel S was used and in the Sta Compact Sta Neoplastine was used. The ISI corresponding to each Reagent /Instrument was used. Statistical analysis was done with Stata 9.0, with correlation coefficient (rho) and a 95% confidence interval; for each case the equation of the straight line was determined. Clinical equivalence was determined through the EP evaluator. Our conclusion is that in patients with liver failure a better statistical and clinical correlation was found for PT%, as it is independent of Time (sec.) and of thromboplastin quality (different concentrations of tissue factor). According to our results, the ISI/INR system is not adequate in patients with liver failure who are candidates for liver transplantation. One of the reasons is that we lack a thromboplastin reagent that is calibrated against plasma of patients with liver failure, as for the ISI calculation plasma of patients with OAC therapy is used (AU)
Subject(s)
Humans , Infant , Child, Preschool , Child , Adolescent , Prothrombin Time , Blood Coagulation Tests/instrumentation , Liver Transplantation , International Normalized Ratio , Hepatic Insufficiency/blood , Prognosis , Cross-Sectional StudiesABSTRACT
La entrada del virus de inmunología humana tipo I (VIH-1) a un linfocito T, CD4+ se inicia por la interacción del trimero viral gp 160, el cual contiene en su extremo la glicoproteína gp120. Esta interaccion directamente con el receptor CD4 del linfocito y posteriormente el complejo CD4-gp 120 reacciona con un correceptor presente en los linfocitos TCD4+. Esta investigación propone un mecanismo de reacción entre la glicoproteína gp120 en su segmento V3 al interactuar con el segundo segmento extracelular en el correceptor CCR5, para ello se han utilizado programas computacionales de enlace proteína-ligando para obtener detalles de las reacciones bioquímicas existentes entre estas regiones, con estos análisis se ha podido explicar la orientación CCR5-gp120 que forma este complejo...
Subject(s)
HIV-1 , Amino Acids , T-Lymphocytes , Acquired Immunodeficiency SyndromeABSTRACT
Background: Infection of the Fallopian tubes (FT) by Neisseria gonorrhoeae (Ngo) can lead to acute salpingitis, an inflammatory condition resulting in damage primarily to the ciliated cells, with loss of ciliary activity and sloughing of the cells from the epithelium. Recently, we have shown that Ngo infection induced apoptosis in FT epithelium cells by a TNF-alpha dependent mechanism that could contribute to the cell and tissue damage observed in gonococcal salpingitis. Aim: To investigate the apoptosis-related genes expressed during apoptosis induction in cultured FT epithelial cells infected in vitro by Ngo. Materials and Methods: In the current study, we used cDNA macroarrays and real time PCR to identify and determine the expression levels of apoptosis related genes during the in vitro gonococci infection of FT epithelial cells. Results: Significant apoptosis was induced following infection with Ngo. Macroarray analysis identified the expression of multiple genes of the TNF receptor family (TNFRSF1B, -4, -6, -10A, -10B and -10D) and the Bcl-2 family (BAK1, BAX, BLK, HRK and MCL-1) without differences between controls and infected cells. This lack of difference was confirmed by RT-PCR of BAX, Bcl-2, TNFRS1A (TNFR-I) and TNFRSF1B (TNFR-II). Conclusion: Several genes related to apoptosis are expressed in primary cultures of epithelial cells of the human Fallopian tube. Infection with Ngo induces apoptosis without changes in the pattern of gene expression of several apoptosis-related genes. Results strongly suggest that Ngo regulates apoptosis in the FT by post-transcriptional mechanisms that need to be further addressed.
Subject(s)
Female , Humans , Apoptosis/genetics , Epithelial Cells/microbiology , Fallopian Tubes/microbiology , Neisseria gonorrhoeae/physiology , Salpingitis/microbiology , Cells, Cultured , Epithelial Cells/pathology , Fallopian Tubes/pathology , Gene Expression Regulation , Oligonucleotide Array Sequence Analysis , /metabolism , Reverse Transcriptase Polymerase Chain Reaction , Receptors, Tumor Necrosis Factor/metabolism , Salpingitis/pathologyABSTRACT
Las enfermedades diarreicas constituyen un problema de salud pública a nivel mundial, especialmente en los países en vías de desarrollo, como Venezuela donde representan unas causa importante de morbilidad y mortalidad infantil. Entre los principales agentes etiológicos bacterianos de esta entidad nosológica se encuentra el género aeromonas, sin embargo, el significado clínico de su aislamiento no se ha establecido definitivamente. El propósito de esta investigación es detectar fenoespecies bacterianas del género aeromonas en muestra de heces de niños aparentemente sanos, con la finalidad de esclarecer la existencia de portadores asintómaticos en nuestro medio y contribuir a dilucidar la importancia clínica de su aislamiento en pacientes con gastroenteritis. La investigación se llevó a cabo durante el período comprendido entre septiembre y diciembre de 1999; se colectaron y analizaron 122 muestras de heces de niños de ambos sexos, con edades comprendidas entre 3 y 6 años, registrados en 16 preescolares privados de la parroquia Chiquiquirá del Municipio Maracaibo. El análisis de las muestras para la detección de fenoespecies del género aeromonas se llevó a cabo empleando la metodología diagnóstica convencional, basada en pruebas bioquímicas. En las muestras analizadas se detectó 27,87 por ciento (34/122) de positividad a especies de aeromonas. Aeromonas caviae resultó la especie más prevalente (52,78 por ciento), seguida de A. jandaei (19,44 por ciento), A. veronii biovar sobria (13,89 por ciento), A. hydrophila (11,11 por ciento) y A. trota (2,78 por ciento). En la mayoría de los casos (94.1 por ciento) se aisló una sola especie por muestra. La distribución de los aislamientos por edad y sexo no resultó estadísticamente significativa (p>0.05). Los resultados obtenidos demuestran la existencia de un número considerable de portadores asintomáticos de aeromonas entre los niños estudiados, por lo que resulta imprescindible realizar mayores investigaciones que contribuyan a establecer el significado de su aislamiento en pacientes con diarrea
Subject(s)
Humans , Male , Female , Child , Aeromonas , Child , Diarrhea, Infantile , Feces , Public Health , Medicine , VenezuelaABSTRACT
El leiomiosarcoma de escroto es un tumor infrecuente. Solo existen 21 casos informados en la literatura. Se realiza el informe de un nuevo caso
Subject(s)
Humans , Male , Aged , Leiomyosarcoma/diagnosis , Leiomyosarcoma/surgery , Scrotum/pathology , Scrotum/surgery , Neoplasm StagingABSTRACT
It can be stated that acceptance of sterilization for limitation of family size has definitely set a new trend in obstetrical practice in this country. It portends the beginning of smaller families in the context of Asian culture and society. Integrating a sterilization program in an OB-GYN Department has demonstrated that it is feasible, practical, effective, and responsive to patient needs. However, since the mean age of our acceptors is 34.2 years and the mean parity is 4.4, future efforts have to be directed to delivery of these services to women of lower age and parity in order to achieve more effective limitation of family size. (Summary)
ABSTRACT
V. flubialis ha sido asociado con diarrea, pero no es muy frecuentemente aislado en nuestro medio. Se reporta un caso de gastroenteritis aguda en un niño de nueve años de edad. Dicho caso resulta inusual debido a que no involucra los probables factores de riesgo epidemiológico. Puesto que se han reportado otros casos en niños, V.fluvialis debe ser considerado en el diagnóstico diferencial de gastroenteritis infantil
Subject(s)
Humans , Male , Child , Diarrhea/diagnosis , Erythrocytes/parasitology , Gastroenteritis/diagnosis , Gastroenteritis/parasitology , Leukocytes/parasitology , Tropical Medicine , VenezuelaABSTRACT
Se presenta el primer caso de Microfilariasis patogena probable Mansonella Ozzardi, diagnosticado por estudio Histopalogico, en un nino de 12 anos de edad, procedente de la ciudad de Santa Cruz, con un cuadro clinico atipico y desenlace fatal
Subject(s)
Humans , Animals , Male , Child , Filariasis/diagnosis , Filariasis/etiology , Filariasis/parasitology , Filariasis/prevention & control , Mansonella/anatomy & histology , Mansonella/growth & development , Mansonella/parasitology , Ceratopogonidae/anatomy & histology , Ceratopogonidae/embryology , Ceratopogonidae/parasitology , Culicidae/isolation & purification , Culicidae/parasitology , Pathology , Pathology/history , Pathology/organization & administration , Pruritus/diagnosis , Pruritus/embryology , Pruritus/parasitology , Pruritus/prevention & controlABSTRACT
Se estudiaron 100 recien nacidos vivos del Hospital Provincial Ginecobstétrico de Cienfuegos que presentaron hipoxia perinatal severa a fin de determinar si en ellos se producían modificaciones morfométricas durante el primer año de vida. Al nacimiento pudo constatarse que el peso y las principales medidas antropométricas de estos niños tienden a ser evidentemente inferiores a las de los neonatos sanos. Posteriormente se aprecia que esos parámetros se acercan a las cifras del percentil 50 y al arribar al primer añode vida, se diferencian poco de los correspondientesa la población que no presentó afectaciones al nacer
Subject(s)
Anthropometry , Asphyxia Neonatorum , InfantABSTRACT
Se realiza un estudio sobre la influencia de la natación en las características métrico funcionales del torax de niños asmáticos en cuatro escuelas primarias de la ciudad de Cienfuegos.Se tomaron 96 niños varones y se dividieron en tres grupos: el grupo 1 a niños sanos, grupo 2 asmáticos, ambos no practicaban de manera sistemática alguna actividad deportiva, y grupo 3 integrado por niños asmáticos que practicaban la natación por indicación médica con un promedio de 2 años en la práctica del deporte.Después de realizar los exámenes correspondientes se determinó que la práctica sistemática de la natación constituye un medio muy positivo para mejorar las condiciones respiratorias de los niños asmáticos
Subject(s)
Swimming , ThoraxSubject(s)
Middle Aged , Humans , Male , Female , Postoperative Complications , Surgical Procedures, Operative/mortality , Risk , Intraoperative ComplicationsABSTRACT
Testosterona foi determinada em amostras de homens normospermicos, oligospermicos e azoospermicos. Foi observado um descrecimo significante em amostras de azoospermicos e oligospermicos. Uma correlacao positiva foi encontrada entre os niveis de testosterona e a motilidade dos espermatozoides
Subject(s)
Humans , Male , Semen , Testosterone , OligospermiaABSTRACT
Os autores se reportam ao estudo radiologico do cancer de mama, atraves de levantamento retrospectivo em 383 casos registrados na Unidade de Mastografia do Instituto Nacional de Ginecologia Preventiva e Reproducao Humana Foi avaliada a frequencia dos sinais primarios e secundarios, analisando-se as caracteristicas de cada um pormenorizadamente. Foi verificada a presenca do sinal primario em 90,3% e constatou-se a presenca de opacidade localizada em 78,6% dos casos. Ausencia de qualquer sinal radiologico que justificasse indicacao de biopsia ocorreu apenas em 4,2% dos casos, constituindo os falso-negativos